Fig 2: EMT Signature identifies an Intermediate subgroup of ovarian carcinoma patients with shorter progression-free survival. (a) Venn diagram of CDH1, ERBB3 and ZEB1 signature showed 36 intersected common transcript IDs. (b) Hierarchical clustering of ovarian carcinoma collection GSE9891 using the 33-gene EMT Signature revealed four clusters Epithelial (red), Intermediate E (blue), Intermediate M (green) and Mesenchymal (purple). (c) Kaplan–Meier Survival plot for PFS at 5 years of GSE9891 collection separated into Epithelial (e), Intermediate E (Int E), Intermediate M (Int M) and Mesenchymal (M) clusters. (d) P-value summary of PFS analysis using log-rank test for paired comparison among four phenotypes. (e) Summary of PFS difference in RMST analysis for paired comparison among four phenotypes

Fig 3: The Intermediate M phenotype is hallmarked by higher N-cadherin and ZEB1 and lower ERBB3 expressions. (a) Plot of QPCR expressions (2^- Avg ?Ct) of CDH2, ITGA5, MMP2 showing the peak expression at Intermediate M (Int M) phenotype. Statistical significance at *P<0.05. (b) IF staining of N-cadherin (N-cad) in Caov3, OVCA432, DOV13 and OVCAR10 representing Epithelial, Intermediate E, Intermediate M and Mesenchymal phenotypes, respectively. Scale bar=200 µm. (c) Distribution of N-cadherin IF positivity among four phenotypes in number (N) and percentage (%). (d) Plot of QPCR expressions (2^- Avg ?Ct) of v-erb-b2 erythroblastic leukaemia viral oncogene homologue 3 (ERBB3) showed lowest expression in the Intermediate M phenotype. Statistical significance at **P<0.05 at both ANOVA and Kruskal–Wallis test. (e) ELISA (f) Plot of QPCR expressions (2^- Avg ?Ct) of snail homologue 1 (SNAI1), zinc-finger E-box-binding homeobox 1 (ZEB1), twist homologue 1 (TWIST1) and zinc-finger E-box-binding homeobox 2 (ZEB2) showing differential expression peaks in Intermediate E (Int E), Intermediate M (Int M) and Mesenchymal (M) phenotypes, respectively. Statistical significance at **P<0.05 in both ANOVA and Kruskal–Wallis tests; Statistical significance at *P<0.05 in either ANOVA or Kruskal–Wallis test